Plasmid

Part:BBa_K5322031:Design

Designed by: Xinquan Zhou   Group: iGEM24_NJTech-China   (2024-10-01)


Programmed Cell Death 1 Ligand 1 Functional Domain [Mus musculus] Expression System


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 377
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 7
    Illegal NheI site found at 30
    Illegal NheI site found at 519
    Illegal PstI site found at 377
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 277
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 377
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 377
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Codon optimization was performed according to the E. coli codon preference table. PD-L1 was truncated, retaining the 1st to 150aa of the original PD-L1 290aa.

Source

Components from BBa_J23119, BBa_B0034, BBa_K5322034, BBa_K731721.

References